Fast and cost effective genome integration with CrEdit

Efficient gene editing tools are essential for studying cellular processes and in engineering organisms for biotechnology and medicine. RNA-guided CRISPR/ Cas9 technology has been used for gene editing in various organisms.

One of the bottlenecks in production of biochemicals and pharmaceuticals in yeast is stable and homogeneous expression of pathway genes.Ronda等。已经开发了一种名为Credit（CRIS/CAS9介导的基因组编辑）的新颖方法，该方法可以快速且具有成本效益的基因组整合来进行酵母菌的工程。S. cerevisiae.

Since the choice of the targeting sites is flexible, CrEdit promises to be a powerful tool for a range of genome engineering applications.

CRISPR/Cas9 in filamentous fungi

在他们的short reportinFungal Biology and Biotechnology, Toru Matsu-ura and his colleagues show that CRISPR/Cas9 technology can be used in a type of red bread mould,Neurosporacrassa,a model organism for filamentous fungi.

使用CRISPR/CAS9技术的有效基因编辑以前尚未显示在该生物体中，并证明该工具可以在其他丝状真菌中使用。

更好地报告更好的研究

‘Reproducibility’ is now increasingly on the radar of funders and is making its rounds in the wider media as well, with concerns of reproducibility making headlines atThe Economist和纽约时报, amongst other outlets. In an effort to enhance the reproducibility of research, BioMed Central launches atrial checklistfor authors and reviewers.

脱氧核糖核酸modifications: 5-mC and beyond

The chemical modification of DNA bases plays a key role in epigenetic gene regulation, in other words; which genes are switched on and off. Methylation of the C base (cytosine) has long been the focus of DNA modification in epigenetic research.

Following on from the recent discovery of methylation of the A base (adenosine) in eukaryotes,Achim Breiling and Frank Lykoreview our current knowledge of DNA modifications with a focus on their regulatory functions.

Sex differences in the ‘landscape’ of the brain in autism

A region of the brain involved in interpreting social situations is unusually smooth in males with autism, but normal in females with the condition. In a new study,Schaer and her colleaguesanalyzed data from theAutism Brain Imaging Data Exchange（Abide），这是来自自闭症患者的大脑图像的开放式存储库，通常会发展男性和女性同龄人。

这使研究人员可以研究106名自闭症患者中的脑褶皱模式，称为Gyri。他们的发现表明，典型的性别差异的某些特征在自闭症患者的大脑中保存下来，而其他人则保留了一些特征。

As part of a specialthematic seriesinMolecular Autism,these findings contribute to the mounting body of evidence for understanding the sex and gender differences in autism.

Molecular insights into Tourette Syndrome

Tourette Syndrome is a highly prevalent childhood neuropsychiatric disorder, characterized by multiple motor and one or more vocal tics.

Rizzo and colleaguessought to determine the transcriptome of circulating miRNAs in individuals with Tourette Syndrome, with their findings revealing circulating miR-429 as a sensitive molecular biomarker that may support the diagnosis of the condition.

It is hoped that this data will pave the way for further molecular characterization and improved diagnosis of Tourette Syndrome in the future.

Bovine viral diarrhea virus

Machine learning is becoming a powerful tool for epidemiological investigation.Gustavo Machado and colleaguesused a random forest prediction model to study BVDV (bovine viral diarrhea virus) occurrence.

他们发现，贫穷或被忽视的繁殖管理（难以控制的因素）需要在BVDV消除工作中进行更多考虑。